Here is the answer to To create a molecule of recombinant DNA, which of the following is cut with a restriction enzyme?
To create a molecule of recombinant DNA, which of the following is cut with a restriction enzyme?
- target DNA
- DNA ligase
- starting DNA
- target RNA
- Below are the answer and explanations.
Restriction enzyme
The restriction enzyme is a class of enzymes found in living organisms. They can cut off foreign DNA, i.e. they can restrict the invasion of foreign DNA and make it inactive. However, they have no damaging effect on their own DNA, which protects the original genetic information of the cell.
Since this cleavage takes place inside the DNA molecule, the name restriction endonucleases. Restriction enzymes are important cutting tools used in genetic engineering. Scientists have isolated many types of restriction enzymes from prokaryotes, and they have been commercialized and widely used in genetic engineering.
According to the characteristics of restriction enzymes cleavage, they can be divided into two major groups: one is non-specific at the cleavage site. The other category is those that can specifically recognize nucleotide sequences, i.e., can only cut at a certain DNA sequence. Such cleavage sites that can be specifically recognized have palindromic sequences, i.e., at the cleavage site. The order of bases in the forward read of one strand is identical to the order of the reverse read of the other strand.
It is the latter type of enzyme that is mostly used in genetic engineering. When restriction enzymes cut at specific cleavage sites, they can be further divided into two types of mismatches and flat cuts according to the way they cut.
A staggered cut is generally a cut at different parts of the two strands. A few nucleotides are separated and the cut ends to form a kind of palindromic single-stranded end, which can be linked to the DNA fragment of the target gene with complementary bases, so it is called sticky end. This enzyme is the most used in genetic engineering.
Another type is to cut at the same part of a specific sequence in both strands to form a flat mouth without a sticky end.
During genetic manipulation, a series of enzymes are used in addition to restriction enzymes to complete the whole process.
Correct Answer
Here is the correct answer to To create a molecule of recombinant DNA, which of the following is cut with a restriction enzyme?
- A and C: target DNA, starting DNA
To create a molecule of recombinant DNA, the target DNA and starting DNA are cut with a restriction enzyme。
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