Here is the answer to The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.
The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.
- Below are the answer and explanations.
Restriction endonuclease
Restriction endonucleases are enzymes that recognize and attach to specific nucleotide sequences and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each strand, referred to as restriction enzymes.
Based on the structure of restriction enzymes, the required cleavage sites of cofactors, and the mode of action, restriction enzymes can be classified into three types, namely, type I, type II, and Type III.
Type I restriction endonucleases catalyze both the methylation of host DNA and the hydrolysis of non-methylated DNA.
While type II restriction endonucleases catalyze the hydrolysis of non-methylated DNA only. Type III restriction endonucleases have both modifying and cognitive cleavage roles.
A class of enzymes is used to recognize a specific deoxyribonucleotide sequence and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each strand.
Restriction enzymes perform interleaved cleavage at the recognition sequence. The result is the formation of two single-stranded ends, which are complementary in nucleotide order and can form hydrogen bonds, so they are called sticky ends, or sticky ends for short.
However, if the cut comes out like a flush end, it is not sticky. In addition, the ends of two cuts with different enzymes are not sticky ends even if they are not flush ends, but the parts that grow out on both sides are not complementary if they are not.
Correct Answer
Here is the correct answer to The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.
- sticky ends
The unpaired nucleotides produced by the action of restriction enzymes are referred to as sticky ends.
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